Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy

@article{Spizzirri2005TimeresolvedCF,
  title={Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy},
  author={Paul G. Spizzirri and J. S. Hill and Stephen B. Kahl and Kenneth P. Ghiggino},
  journal={Lasers in Medical Science},
  year={2005},
  volume={11},
  pages={237-246}
}
The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution. The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP… 
1 Citations
Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers.
TLDR
A maximum useful porphyrin concentration for fluorescence imaging purposes of approximately 30 microg mL(-1) is revealed.

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