Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy

@article{Spizzirri2005TimeresolvedCF,
  title={Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy},
  author={Paul G. Spizzirri and J. S. Hill and Stephen B. Kahl and Kenneth P. Ghiggino},
  journal={Lasers in Medical Science},
  year={2005},
  volume={11},
  pages={237-246}
}
The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution. The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP… 
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Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers.
TLDR
A maximum useful porphyrin concentration for fluorescence imaging purposes of approximately 30 microg mL(-1) is revealed.

References

SHOWING 1-10 OF 45 REFERENCES
Intracellular fluorescence polarization, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins
Porphyrin monomers, dimers, and aggregates with various tumor- localizing and photosensitizing properties can be differentiated based on their fluorescence lifetimes. These individual components are…
Time-resolved polarization measurements of porphyrin fluorescence in solution and in single cells.
Photophysics and Intracellular Distribution of a Boronated Porphyrin Phototherapeutic Agent
TLDR
Time‐resolved confocal fluorescence imaging of the intracellular distribution of BOPP in C6 glioma cells indicates distinct subcellular localization and heterogeneity of emission, which is interpreted and discussed in terms of the possible mechanisms for cellular uptake and localization.
Time-resolved fluorescence in photobiology.
Fluorescence lifetime measurements under a microscope by the time-correlated single-photon counting technique
SUBCELLULAR LOCALIZATION OF PORPHYRINS USING CONFOCAL LASER SCANNING MICROSCOPY
The in vitro subcellular distribution patterns of 10 porphyrins, varying in hydrophobicity and charge, were studied using confocal laser scanning microscopy on two cell lines (V79 and C6 glioma…
PHOTOCHEMICAL PROPERTIES OF PORPHYRIN c: AN AGENT FOR USE IN TUMOR PHOTOTHERAPY
TLDR
Both Pc and Zn‐Pc are effective agents in tumor phototherapy and do not induce the prolonged cutaneous photosensitivity observed with the use of HpD.
Luminescence lifetimes in biological systems.
TLDR
Modern laser-based techniques for the measurement of fluorescence decay times in the nanosecond to picosecond time-domain, time-gated fluorescence spectra, and fluorescence anisotropy, are outlined, with recent results on several biological systems with both intrinsic and extrinsic probes used as examples.
Picosecond time-resolved fluorescence spectroscopy of hematoporphyrin derivative
The picosecond time-resolved fluorescence decays I(t) and spectra I(\lambda, t) for hematoporphyrin derivative (HPD) in a phosphate buffer saline aqueous solution at different concentrations ( 8.4…
Fluorescence lifetime measurements using a novel fiber‐optic laser scanning confocal microscope
The novel design of a fiber‐optic laser scanning confocal microscope is described. The optical fiber acts as a flexible light path for both the excitation and return beams and as the confocal pinhole…
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