Time-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing.

@article{Welle2016TimeresolvedAO,
  title={Time-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing.},
  author={Kevin A. Welle and Tian Zhang and Jennifer R. Hryhorenko and Shichen Shen and Jun Qu and Sina Ghaemmaghami},
  journal={Molecular & cellular proteomics : MCP},
  year={2016},
  volume={15 12},
  pages={3551-3563}
}
Recent advances in mass spectrometry have enabled system-wide analyses of protein turnover. By globally quantifying the kinetics of protein clearance and synthesis, these methodologies can provide important insights into the regulation of the proteome under varying cellular and environmental conditions. To facilitate such analyses, we have employed a methodology that combines metabolic isotopic labeling (Stable Isotope Labeling in Cell Culture - SILAC) with isobaric tagging (Tandem Mass Tags… CONTINUE READING
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