Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM.

@article{Haga2000TimelapseVI,
  title={Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM.},
  author={Hisashi Haga and Masafumi Nagayama and Kazushige Kawabata and Eisuke Ito and Tatsuo Ushiki and T Sambongi},
  journal={Journal of electron microscopy},
  year={2000},
  volume={49 3},
  pages={473-81}
}
Using the force modulation mode in atomic force microscopy, we have succeeded in capturing time-lapse viscoelastic images of living mouse fibroblasts (NIH3T3) for several hours in a physiological condition without damaging the fibroblasts. Elongation of the lamellipodia and swelling of blebs were observed in time-lapse topographic images, which were taken every 10 min. The corresponding viscoelastic responses at a frequency of 600 Hz were visualized as consecutive images. The stiffer part of… CONTINUE READING

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