Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies.

@article{Okuda2003TimedependentCF,
  title={Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies.},
  author={Tatsuya Okuda and Satoru Kidoaki and Mio Ohsaki and Yoshiyuki Koyama and Kenichi Yoshikawa and Takuro Niidome and Haruhiko Aoyagi},
  journal={Organic & biomolecular chemistry},
  year={2003},
  volume={1 8},
  pages={1270-3}
}
Dendritic poly(L-lysine) of the 6th generation shows high transfection efficiency into several cultivated cells with low cytotoxicity. In order to understand the mechanism of complex formation with plasmid DNA, the complex was observed using atomic force microscopy. After mixing for 15 min, 1-2 microns assemblies of complexes composed of several small particles (50-200 nm) were observed. At the same time, individual small complexes of 50 to 500 nm were observed on a mica surface. After… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…