Time course of meiotic progression after transferring primary spermatocyte into ooplasm at different stages.

@article{Nan2007TimeCO,
  title={Time course of meiotic progression after transferring primary spermatocyte into ooplasm at different stages.},
  author={C L Nan and Ying-Chun Ouyang and Zhen-Jun Zhao and Yan Jiang and Zi-Li Lei and Jun-Cheng Huang and Xiang-Fen Song and Qing-Yuan Sun and Da-Yuan Chen},
  journal={Molecular reproduction and development},
  year={2007},
  volume={74 9},
  pages={1072-80}
}
This study attempted to investigate the time course of meiotic progression after transferring primary spermatocyte (PS) into ooplasm at different maturing stages. In present experiments, PSs were introduced into maturing ooplasts or oocytes by electrofusion. Higher fusion rate was obtained by phytohemagglutinin (PHA) agglutination than by perivitelline space (PVS) insertion. When the ooplasms prepared at 0, 2, 5, and 8.5 hr of in vitro maturation (IVM) were used as recipients and PSs were used… CONTINUE READING