Time and force dependence of the rupture of glycoprotein IIb-IIIa-fibrinogen bonds between latex spheres.

@article{Goldsmith2000TimeAF,
  title={Time and force dependence of the rupture of glycoprotein IIb-IIIa-fibrinogen bonds between latex spheres.},
  author={Harry L. Goldsmith and Fiona A. McIntosh and Jack Shahin and Mony M. Frojmovic},
  journal={Biophysical journal},
  year={2000},
  volume={78 3},
  pages={1195-206}
}
We studied the shear-induced breakup of doublets of aldehyde/sulfate (A/S) latex spheres covalently linked with purified platelet GPIIb-IIIa receptor, and cross-linked by fibrinogen. Flow cytometry with fluorescein isothiocyanate-fibrinogen showed than an average of 22,500 molecules of active GPIIb-IIIa were captured per sphere, with a mean K(d) = 56 nM for fibrinogen binding. The spheres, suspended in buffered 19% Ficoll 400 containing 120 or 240 pM fibrinogen, were subjected to Couette flow… CONTINUE READING