Time and dose-related ultraviolet B damage in viable pig skin explants held in a newly developed organ culture system.

@article{Rijnkels2001TimeAD,
  title={Time and dose-related ultraviolet B damage in viable pig skin explants held in a newly developed organ culture system.},
  author={Jolanda M Rijnkels and Laurence O. Whiteley and Gerard M J Beijersbergen van Henegouwen},
  journal={Photochemistry and photobiology},
  year={2001},
  volume={73 5},
  pages={
          499-504
        }
}
For facilitating photochemical and toxicological studies an ex vivo skin model was developed in our laboratory using skin from domestic pigs. The model comprised the use of a complete skin piece, including the dermis and stratum corneum, of bigger areas to make future topical applications easier. Fully differentiated skin explants (5 x 50 mm, thickness 5 mm) were irradiated with ultraviolet B (UVB; 1-10 kJ/m2; 6 W/m2). Directly thereafter they were brought in culture (Dulbeccos modified Eagles… CONTINUE READING
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