Tilted view reconstruction in optical microscopy. Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei.

@article{Shaw1989TiltedVR,
  title={Tilted view reconstruction in optical microscopy. Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei.},
  author={Peter J. Shaw and David A. Agard and Yasushi Hiraoka and John Sedat},
  journal={Biophysical journal},
  year={1989},
  volume={55 1},
  pages={101-10}
}
The resolution along the optical axis (z) is much less than the in-plane resolution in any current optical microscope, conventional or otherwise. We have used mutually tilted, through-focal section views of the same object to provide a solution to this problem. A tilting specimen stage was constructed for an optical microscope, which with the use of a coverslip-free water immersion lens, allowed the collection of data sets from intact Drosophila melanogaster embryos at viewing directions up to… CONTINUE READING