Tigliane diterpenes from Croton mauritianus as inhibitors of chikungunya virus replication.

Abstract

A bioassay-guided purification of an EtOAc extract of the leaves of Croton mauritianus using a chikungunya virus-cell-based assay led to the isolation of 12-O-decanoylphorbol-13-acetate (1) and the new 12-O-decanoyl-7-hydroperoxy-phorbol-5-ene-13-acetate (2), along with loliolide, vomifoliol, dehydrovomifoliol, annuionone D and bluemol C. The planar structure and the relative configuration of compound 2 were elucidated based on spectroscopic analysis, including 1D- and 2D-NMR experiments, mass spectrometry, and comparison with literature data. Compounds 1 and 2 inhibited chikungunya virus-induced cell death in cell culture with EC50s of 2.4±0.3 and 4.0±0.8 μM, respectively.

DOI: 10.1016/j.fitote.2014.05.015

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Cite this paper

@article{Corlay2014TiglianeDF, title={Tigliane diterpenes from Croton mauritianus as inhibitors of chikungunya virus replication.}, author={Nina Corlay and Leen Delang and Emmanuelle Girard-Valenciennes and Johan Neyts and Patricia Clerc and Jacqueline Smadja and Françoise Gu{\'e}ritte and Pieter Leyssen and Marc Litaudon}, journal={Fitoterapia}, year={2014}, volume={97}, pages={87-91} }