Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments

@article{Prewitz2013TightlyAT,
  title={Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments},
  author={Marina C Prewitz and F Philipp Seib and Malte von Bonin and Jens Friedrichs and Aline Sti\ssel and Christian Niehage and Katrin B. M{\"u}ller and Konstantinos Anastassiadis and Claudia Waskow and Bernard Hoflack and Martin Bornhaeuser and Carsten Werner},
  journal={Nature Methods},
  year={2013},
  volume={10},
  pages={788-794}
}
A major obstacle in defining the exact role of extracellular matrix (ECM) in stem cell niches is the lack of suitable in vitro methods that recapitulate complex ECM microenvironments. Here we describe a methodology that permits reliable anchorage of native cell–secreted ECM to culture carriers. We validated our approach by fabricating two types of human bone marrow–specific ECM substrates that were robust enough to support human mesenchymal stem cells (MSCs) and hematopoietic stem and… CONTINUE READING
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