Tight ligand binding affinities determined from thermodynamic linkage to temperature by titration calorimetry.

@article{Doyle1998TightLB,
  title={Tight ligand binding affinities determined from thermodynamic linkage to temperature by titration calorimetry.},
  author={Michelle Doyle and Preston Hensley},
  journal={Methods in enzymology},
  year={1998},
  volume={295},
  pages={88-99}
}
A general isothermal titration calorimetry method is described that can be used to determine equilibrium binding constants for high-affinity interactions of ligands with biological macromolecules. The method exploits the thermodynamic linkage between the ligand binding equilibrium constant and temperature. By measuring the binding enthalpy change for an… CONTINUE READING