Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods.

@article{Miller2003TightJM,
  title={Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods.},
  author={Daniel S. J. Miller and Judith J. Eckert and Giovanna Lazzari and Veronique Duranthon-Richoux and Joe Sreenan and Dermot Morris and Cesare Galli and Jean-Paul Renard and Tom P. Fleming},
  journal={Biology of reproduction},
  year={2003},
  volume={68 4},
  pages={1394-402}
}
We have established a transcription map of individual bovine embryos using semiquantitative reverse transcriptase-polymerase chain reaction to detect the levels of six marker genes involved in early embryo differentiation. The critical step of compaction during preimplantation development is often not accomplished or it takes place for only a short period in in vitro generated embryos, which may result in reduced viability. Compaction is accompanied by the assembly of intercellular tight… CONTINUE READING