Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2s-M2 tetracycline transactivator and repressor system

@article{Salucci2002TightCO,
  title={Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2s-M2 tetracycline transactivator and repressor system},
  author={Valentina Salucci and Alessia Scarito and Luigi Aurisicchio and Stefania Lamartina and Giancarlo Nicolaus and Saverio Giampaoli and Odalys Gonzalez-Paz and Carlo Toniatti and Hermann Bujard and Wolfgang Hillen and Gennaro Ciliberto and Fabio Palombo},
  journal={Gene Therapy},
  year={2002},
  volume={9},
  pages={1415-1421}
}
Control of gene expression for gene therapy application requires the design of a sophisticated system embodying multiple properties. The ideal system should present the following features: (1) low or undetectable gene expression in the absence of inducer; (2) strong expression upon induction; and (3) fast kinetics of induction in the presence of inducers and rapid reversal of induction after its withdrawal. To evaluate these parameters, the features of the latest generation tetracycline… CONTINUE READING