Tight control of exogenous SERCA expression is required to obtain acceleration of calcium transients with minimal cytotoxic effects in cardiac myocytes.

@article{ODonnell2001TightCO,
  title={Tight control of exogenous SERCA expression is required to obtain acceleration of calcium transients with minimal cytotoxic effects in cardiac myocytes.},
  author={James M. O'Donnell and Carlota M Sumbilla and Hongling Ma and Iain K. G. Farrance and M Cavagna and Michael G. Klein and Giuseppe Inesi},
  journal={Circulation research},
  year={2001},
  volume={88 4},
  pages={415-21}
}
Collateral effects of exogenous sarcoendoplasmic reticulum Ca(2+) ATPase (SERCA) expression were characterized in neonatal rat and chicken embryo cardiac myocytes, and the conditions required to produce acceleration of Ca(2+) transients with minimal toxicity were established. Cultured myocytes were infected with adenovirus vector carrying the cDNA of wild-type SERCA1, an inactive SERCA1 mutant, or enhanced green fluorescence protein under control of the cytomegalovirus promoter. Controls were… CONTINUE READING