Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae.

@article{Dam1999TickborneRF,
  title={Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae.},
  author={Alje P. van Dam and Tom van Gool and Jos{\'e} C F M Wetsteyn and Jeanette Dankert},
  journal={Journal of clinical microbiology},
  year={1999},
  volume={37 6},
  pages={2027-30}
}
West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fever of unknown origin by quantitative buffy coat (QBC) analysis. Diagnosis was confirmed by intraperitoneal inoculation of blood specimens from patients into laboratory mice. In… CONTINUE READING