Thyrotropin receptors on bovine thyroid membranes: two types with different affinities and specificities.

@article{Pekonen1979ThyrotropinRO,
  title={Thyrotropin receptors on bovine thyroid membranes: two types with different affinities and specificities.},
  author={Fredrika Pekonen and Bruce D. Weintraub},
  journal={Endocrinology},
  year={1979},
  volume={105 2},
  pages={
          352-9
        }
}
We have studied the effects of pH, temperature, and ionic strength on the characteristics of bovine TSH (bTSH) binding to bovine thyroid membranes. Although maximal bTSH binding occurred at low pH, temperature, and ionic strength, the maximal sensitivity [defined as the amount of bTSH yielding 50% inhibition of tracer binding (B/Bo = 50%)] of the assay was observed at physiological pH and temperature in the presence of 50 mM NaCl. Under the latter conditions, the TSH binding assay was highly… CONTINUE READING