Thyroid hormone is a critical determinant for the regulation of the cochlear motor protein prestin

@article{Weber2002ThyroidHI,
  title={Thyroid hormone is a critical determinant for the regulation of the cochlear motor protein prestin},
  author={Thomas Weber and U. Zimmermann and H. Winter and A. Mack and I. Köpschall and K. Rohbock and H. Zenner and M. Knipper},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99},
  pages={2901 - 2906}
}
The most impressive property of outer hair cells (OHCs) is their ability to change their length at high acoustic frequencies, thus providing the exquisite sensitivity and frequency-resolving capacity of the mammalian hearing organ. Prestin, a protein related to a sulfate/anion transport protein, recently has been identified and proposed as the OHC motor molecule. Homology searches of 1.5 kb of genomic DNA 5′ of the coding region of the prestin gene allowed the identification of a thyroid… Expand
Thyroid hormone receptor α1 is a critical regulator for the expression of ion channels during final differentiation of outer hair cells
TLDR
The data support the hypothesis that proteins expressed in rodents prior to or briefly after birth like CaV1.3 and prestin are either independent of TH or enhanced through TRβ, and that the precise regulation of expression of the latter genes requires a critical local TH level to overcome the TRα1 repression. Expand
Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells
TLDR
The cloning and characterization of the complete genomic structure of the mouse Prestin (mPres) gene is reported and it is concluded that the mPres gene is centromerically related to and resides within 19 kb of, the Reln gene. Expand
The Motor Protein Prestin Is a Bullet-shaped Molecule with Inner Cavities*
TLDR
The square-shaped top view of purified prestin, the binding of multiple anti-FLAG antibodies to each prestin particle, the native-PAGE analysis, and the much larger molecular weight obtained from size exclusion chromatography than the estimation for the monomer all support that prestin is a tetramer. Expand
Prestin Contributes to Membrane Compartmentalization and Is Required for Normal Innervation of Outer Hair Cells
TLDR
It is indicated that prestin, independent of its motile function, plays an important structural role in membrane compartmentalization, which is required for the formation of normal efferent-OHC synapses in mature OHCs. Expand
Analysis of the Oligomeric Structure of the Motor Protein Prestin*
TLDR
Chemical cross-linking experiments, perfluoro-octanoate-PAGE/Western blot, and affinity purification experiments all indicate that prestin exists as a higher order oligomer, such as a tetramer, in prestin-expressing yeast, mammalian cell lines and native outer hair cells. Expand
Developmental roles of the thyroid hormone receptor α and β genes
TLDR
The deletion of all α and β receptors results in viable mice, but these exhibit immaturity in multiple systems, indicating the importance of T3 signaling at the later, maturational stages of development. Expand
Developmental Expression of the Outer Hair Cell Motor Prestin in the Mouse
TLDR
Development of motor protein activity in the lateral membrane of the mouse outer hair cell from postnatal day 5 to P18 was investigated under whole-cell voltage clamp and it is suggested that molecular motors mature to augment nonlinear capacitance. Expand
Thyroid hormone receptors TRα1 and TRβ differentially regulate gene expression of Kcnq4 and prestin during final differentiation of outer hair cells
TLDR
It is shown that T3 target genes can differ in their sensitivity to TH receptors having the ligand either bound (holoreceptor) or not bound (aporeceptors) within single cells, and a role for TRα1 in final cell differentiation is suggested. Expand
A protective role for type 3 deiodinase, a thyroid hormone-inactivating enzyme, in cochlear development and auditory function.
TLDR
The results indicate a protective role for type 3 deiodinase in hearing, and the auditory system illustrates the considerable extent to which tissues can autoregulate their developmental response to thyroid hormone through both type 2 and 3 deodinases. Expand
Generation of somatic electromechanical force by outer hair cells may be influenced by prestin–CASK interaction at the basal junction with the Deiter’s cell
TLDR
It is demonstrated that this force is significantly reduced when the immature subcellular distribution of prestin found along the entire plasma membrane persists into maturity, as has been described in previous studies under hypothyroidism. Expand
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