Thymine auxotrophy as an attenuating marker in Vibrio cholerae.

  title={Thymine auxotrophy as an attenuating marker in Vibrio cholerae.},
  author={Stephen R. Attridge},
  journal={Microbial pathogenesis},
  volume={19 1},
Vibrio cholerae CVD102 is a thymine-dependent auxotroph of CVD101, a cholera toxin A-B+ candidate live oral cholera vaccine. Previous clinical experience with these strains suggested that, by restricting intestinal growth, thymine auxotrophy is attenuating for V. cholerae. Studies in the infant mouse cholera model cast doubt upon this conclusion however. Stable thyA mutants selected from each of three pathogenic strains showed unimpaired gut colonization in mixed-infection competition… CONTINUE READING