Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes.

@article{MunierLehmann2001ThymidylateKO,
  title={Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes.},
  author={H{\'e}l{\'e}ne Munier-Lehmann and Alain F. Chaffotte and Sylvie Pochet and Gilles Labesse},
  journal={Protein science : a publication of the Protein Society},
  year={2001},
  volume={10 6},
  pages={1195-205}
}
We have overexpressed in Escherichia coli the thymidylate kinase of Mycobacterium tuberculosis (TMPKmt). Biochemical and physico-chemical characterization of TMPKmt revealed distinct structural and catalytic features when compared to its counterpart from yeast (TMPKy) or E. coli (TMPKec). Denaturation of the dimeric TMPKmt by urea under equilibrium conditions was studied by intrinsic fluorescence and circular dichroism (CD) spectroscopy. It suggested a three-state unfolding mechanism with a… CONTINUE READING

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