Thymidine phosphorylase activity of anucleate minicells of Escherichia coli immobilized in an agarose gel matrix


Escherichia coli whole cells, and minicells were immobilized on columns in an agarose gel matrix and evaluated for their thymidine phosphorylase (TPP) activity. The greatest specific activity of TPP was found with the induced minicells (2.2 μmoles thymine/min/mg protein) at a column retention time of 11 min. 
DOI: 10.1007/BF00134669


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