Thymidine kinase, thymidylate synthase, and endothelial cell growth under hyperoxia.

  title={Thymidine kinase, thymidylate synthase, and endothelial cell growth under hyperoxia.},
  author={Alain F. Junod and Heidi Petersen and Lan Jornot},
  journal={Journal of applied physiology},
  volume={62 1},
To determine the respective role of thymidine kinase and thymidylate synthase activities in the hyperoxia-induced decrease in DNA synthesis and their relationship with cell replication, we measured these two enzyme activities in primary cultures of porcine aortic endothelial cells under different O2 concentrations for various durations. In confluent cells, exposure to 95% O2 for 5 days reduced thymidine kinase activity to 15% of control values; thymidylate synthase activity was unaffected. In… CONTINUE READING

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