Thymidine 2'-hydroxylation in Neurospora crassa.

  title={Thymidine 2'-hydroxylation in Neurospora crassa.},
  author={L. Bankel and G. Lindstedt and S. Lindstedt},
  journal={The Journal of biological chemistry},
  volume={247 19},
Abstract An enzyme preparation catalyzing the 2'-hydroxylation of thymidine was prepared from mycelial extracts of Neurospora crassa strain STA 4 (FGSC 262A) by chromatography on hydroxylapatite and DEAE-Sephadex A-50. The enzyme had an isoelectric point of 4.6 and a molecular weight of 47,000 (determined by gel filtration). The reaction product was identical with thymine riboside on ion exchange and thin layer chromatography. Paper chromatography after reduction and hydrolysis showed that the… Expand
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