Thymidine 2'-hydroxylation in Neurospora crassa.

  title={Thymidine 2'-hydroxylation in Neurospora crassa.},
  author={L Bankel and G{\"o}ran Lindstedt and Sven Lindstedt},
  journal={The Journal of biological chemistry},
  volume={247 19},
An enzyme preparation catalyzing the 2’-hydroxylation of thymidine was prepared from mycelial extracts of Neurospora crassa strain STA 4 (FGSC 2628) by chromatography on hydroxylapatite and DEAE-Sephadex A-50. The enzyme had an isoelectric point of 4.6 and a molecular weight of 47,000 (determined by gel filtration). The reaction product was identical with thymine riboside on ion exchange and thin layer chromatography. Paper chromatography after reduction and hydrolysis showed that the pentose… CONTINUE READING