Thylakoid membrane at altered metabolic state: challenging the forgotten realms of the proteome.

@article{Rexroth2003ThylakoidMA,
  title={Thylakoid membrane at altered metabolic state: challenging the forgotten realms of the proteome.},
  author={Sascha Rexroth and J{\"u}rgen M W Meyer zu Tittingdorf and Frank Krause and Norbert A. Dencher and Holger Seelert},
  journal={Electrophoresis},
  year={2003},
  volume={24 16},
  pages={2814-23}
}
Analysis of the membrane integral proteome is mainly dependent on the ability of protein separation. Blue-native polyacrylamide gel electrophoresis (BN-PAGE) is a technique capable of efficient membrane protein separation, so far mainly applied to the mitochondrial oxidative phosphorylation machinery. Applying BN-PAGE to the thylakoid membranes after mild solubilization with digitonin we succeeded in displaying the response of the green algae Chlamydomonas reinhardtii to altered culture… CONTINUE READING
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