Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3.

@article{Kobayashi1996ThrombopoietinSP,
  title={Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3.},
  author={Minoru Kobayashi and Joseph H. Laver and Takashi Kato and Hiroshi Miyazaki and Makio Ogawa},
  journal={Blood},
  year={1996},
  volume={88 2},
  pages={429-36}
}
We have studied the effects of recombinant human thrombopoietin (TPO; mpl ligand) on the proliferation of human primitive hematopoietic progenitors in vitro. CD34+ cells were enriched for cell-cycle-dormant primitive progenitors by separation on the basis of expression of c-kit and CD38. In the presence of varying combinations of TPO, Steel factor (SF), and interleukin-3 (IL-3), CD34+/c-kit(low)/CD38neg/low cells produced fewer colonies than CD34+/c-kit(low)/CD38high cells. However, when… CONTINUE READING