Thrombomodulin activity on mesothelial cells: perspectives for mesothelial cells as an alternative for endothelial cells for cell seeding on vascular grafts

  title={Thrombomodulin activity on mesothelial cells: perspectives for mesothelial cells as an alternative for endothelial cells for cell seeding on vascular grafts},
  author={Hendricus Martinus Christianus Verhagen and G J Heijnen-Snyder and Apollo Pronk and Thea M. Vroom and Th. J. M. V. van Vroonhoven and Bert C. Eikelboom and Jan J. Sixma and PhG de Groot},
  journal={British Journal of Haematology},
Lining the luminal surface of prosthetic small diameter bypasses with endothelial cells (EC) will lower its thrombogenicity. Unfortunately, human EC are scarce. Mesothelial cells (MC) may be a valuable alternative for EC, since they are abundantly available and have antithrombotic and fibrinolytic properties. An important anticoagulant function of EC is due to thrombomodulin (TM) on the surface. The presence of TM on omentally derived human MC is not known but would increase the chance of… 

Human mesothelial cells express tissue factor when switched to proliferating state. Pharmacological modulation in vitro

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The results suggest that endoluminal seeding of syngeneic SMCs can be effective in reducing intimal hyperplasia both in a deendothelialization model and in arterial allografts.

Novel vascular graft grown within recipient's own peritoneal cavity.

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In vivo experiments with mesothelial cell seeded ePTFE vascular grafts.

Vascular grafts and the endothelium.

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It is concluded that human peritoneal mesothelial cells may be used for cell seeding procedures, provided that their tissue factor expression can be controlled.

A study of the possible role of mesothelium as a surface for flowing blood.

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Tissue factor expression on mesothelial cells is induced during in vitro culture--manipulation of culture conditions creates perspectives for mesothelial cells as a source for cell seeding procedures on vascular grafts.

Conditions in which Mesothelial cells are a good alternative for EC for seeding on synthetic grafts are established, established by reducing TF expression by 90%, are established.

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Thrombogenicity of vascular cells. Comparison between endothelial cells isolated from different sources and smooth muscle cells and fibroblasts.

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Thrombomodulin expression in malignant pleural mesothelioma and pulmonary adenocarcinoma.

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The origins, nature, and reactions of the mesothelium have intrigued investigators for over 100 years, and some of its complexities as viewed by embryologists, electronmicroscopists, cell biologists, pathologists, and oncologists are attempted.

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Effects of autologous mesothelial cell seeding on prostacyclin production within Dacron® arterial prostheses

Preliminary results, showing that grafts seeded with autologous peritoneal mesothelial cells release more prostacyclin than unseeded grafts, further highlight the role of the Mesothelial cell as an alternative to the endothelial cell for improving the patency of arterial Dacron prostheses in the early postoperative days.