Thrombin and phorbol esters cause the selective phosphorylation of a G protein other than Gi in human platelets.

@article{Carlson1989ThrombinAP,
  title={Thrombin and phorbol esters cause the selective phosphorylation of a G protein other than Gi in human platelets.},
  author={Kenneth E. Carlson and Lawrence F Brass and David R Manning},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 22},
  pages={13298-305}
}
Preincubation of human platelets with activators of protein kinase C such as phorbol 12-myristate 13-acetate (PMA) has been shown previously to attenuate the ability of agonists both to suppress formation of cAMP and to stimulate hydrolysis of phosphoinositides. In the present study, we have examined whether the attenuation caused by PMA can be attributed to the phosphorylation of the alpha subunit(s) of Gi, a GTP-binding regulatory protein implicated in several pathways of signal transduction… CONTINUE READING