Thrombin and NaF, but not epinephrine, raise cytosolic free Na+ in human platelets.

@article{Stamouli1993ThrombinAN,
  title={Thrombin and NaF, but not epinephrine, raise cytosolic free Na+ in human platelets.},
  author={V Stamouli and Catherine Vakirtzi-Lemonias and Winfried Siffert},
  journal={Biochimica et biophysica acta},
  year={1993},
  volume={1176 3},
  pages={215-21}
}
We have investigated changes in [Na+]i in SBFI-loaded platelets stimulated at 37 degrees C with thrombin, epinephrine, and NaF. Basal [Na+]i was 4.9 +/- 1.3 mM (n = 70). Stimulation of platelets with thrombin (0.1 U/ml) in the presence of 1 mM extracellular Ca2+ rapidly raised [Na+]i by 27.3 +/- 6 mM (n = 16). Part of this increase (approx. 20-30%) is caused by Na+/H+ exchange, the rest is predominantly due to Na+ influx. Epinephrine (20 microM) failed to change [Na+]i both in the absence and… CONTINUE READING