Three putative N‐glycosylation sites within the murine 5‐HT3A receptor sequence affect plasma membrane targeting, ligand binding, and calcium influx in heterologous mammalian cells

@article{Quirk2004ThreePN,
  title={Three putative N‐glycosylation sites within the murine 5‐HT3A receptor sequence affect plasma membrane targeting, ligand binding, and calcium influx in heterologous mammalian cells},
  author={Phillip L. Quirk and Suma Rao and Bryan L. Roth and Ruth Elise Siegel},
  journal={Journal of Neuroscience Research},
  year={2004},
  volume={77}
}
The serotonin type 3A receptor (5‐HT3AR) is a ligand‐gated ion channel (LGIC) that modulates a diverse set of cognitive and physiological functions. The 5‐HT3AR, as with other LGICs, is a pentameric ion channel comprising five glycoprotein subunits. Although the N‐terminal of the 5‐HT3AR contains three putative N‐linked glycosylation sites, the importance of each glycosylation site has not yet been established. To address this question, we used tunicamycin treatment and site‐directed… 
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TLDR
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Role of N-Linked Glycosylation of the 5-HT2A Receptor in JC Virus Infection
TLDR
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Thermal Unfolding of a Mammalian Pentameric Ligand-gated Ion Channel Proceeds at Consecutive, Distinct Steps*
TLDR
The thermal unfolding of a homopentameric LGIC, the 5-hydroxytryptamine receptor, was analyzed in plasma membranes as well as during detergent extraction, purification, and reconstitution into artificial lipid bilayers, finding a large loss in thermostability correlating with the loss of the lipid bilayer during membrane solubilization and purification.
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