Three-phase solvent systems for comprehensive separation of a wide variety of compounds by high-speed counter-current chromatography.

@article{Shibusawa2006ThreephaseSS,
  title={Three-phase solvent systems for comprehensive separation of a wide variety of compounds by high-speed counter-current chromatography.},
  author={Yoichi Shibusawa and Yutaka Yamakawa and Ryoko Noji and Akio Yanagida and Heisaburo Shindo and Yoichiro Ito},
  journal={Journal of chromatography. A},
  year={2006},
  volume={1133 1-2},
  pages={119-25}
}
Three-phase solvent systems were efficiently utilized for high-speed counter-current chromatography (HSCCC) to separate multiple components with a wide range of hydrophobicity. The compositions of three-phase systems were optimized according to their physical parameters such as volume ratio, viscosity and specific gravity of upper (UP), middle (MP) and lower (LP) phases. The three-phase systems composed of n-hexane-methyl acetate-acetonitrile-water (4:4:3:4, v/v/v/v) was selected for HSCCC… CONTINUE READING