Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties.

  title={Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties.},
  author={Naoki Itano and Takahiro Sawai and Masahiro Yoshida and Petros Lenas and Yukio Yamada and M. Imagawa and Tamayuki Shinomura and Masaaki Hamaguchi and Yasukazu Yoshida and Yoshiki Ohnuki and Seiji Miyauchi and Andrew P. Spicer and John Alan McDonald and Koji Kimata},
  journal={The Journal of biological chemistry},
  volume={274 35},
Three mammalian hyaluronan synthase genes, HAS1, HAS2, and HAS3, have recently been cloned. In this study, we characterized and compared the enzymatic properties of these three HAS proteins. Expression of any of these genes in COS-1 cells or rat 3Y1 fibroblasts yielded de novo formation of a hyaluronan coat. The pericellular coats formed by HAS1 transfectants were significantly smaller than those formed by HAS2 or HAS3 transfectants. Kinetic studies of these enzymes in the membrane fractions… CONTINUE READING
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