Three genes specifically expressed during phosphate deficiency in Pholiota nameko strain N2 encode hydrophobins

  title={Three genes specifically expressed during phosphate deficiency in Pholiota nameko strain N2 encode hydrophobins},
  author={Yuji Tasaki and Koji Ohata and Takashi Hara and Toshio Joh},
  journal={Current Genetics},
Abstract We previously isolated 31 cDNAs corresponding to pdi [inorganic phosphate (Pi) deficiency-inducible] genes through the differential screening of a cDNA library constructed from the mycelium of Pholiota nameko strain N2 cultured in Pi-depleted medium. Among the cDNAs, pdi251, pdi263 and pdi315 were analyzed here. The deduced amino acid sequences of pdi251, pdi263 and pdi315 showed high similarity to fungal hydrophobins and genes corresponding to these cDNAs encoding P. nameko… 

TasHyd1, a new hydrophobin gene from the biocontrol agent Trichoderma asperellum, is involved in plant root colonization.

TasHyd1 deletion mutants had no significant reduction in in vitro mycoparasitic activity but were altered in their wettability and were severely impaired in root attachment and colonization, indicating that the protein is a new hydrophobin that contributes to Trichoderma interaction with the plant.

Evolutionary analysis of hydrophobin gene family in two wood-degrading basidiomycetes, Phlebia brevispora and Heterobasidion annosum s.l.

The phylogenetic analysis confirmed the important role of gene duplication events in the evolution of hydrophobins in basidiomycetes and revealed significant differences in the expression levels of the 16 H. annosum s.l. hydrophobin-encoding genes which suggest possible differences in their regulatory patterns.

Comparative genomics and expression levels of hydrophobins from eight mycorrhizal genomes

Hydrophobin gene regulation in mycorrhizal tissue as compared with free-living mycelium, however, was significantly associated with a calculated high exposure of hydrophilic residues.

Characterization of a Pleurotus ostreatus fruiting body‐specific hydrophobin gene, Po.hyd

The putative Po.HYD protein is assigned to the classI hydrophobins since its sequence arrangement and hydropathy pattern has a high consensus to other known class I hydrophobic proteins.

Quo vadis: signaling molecules and small secreted proteins from mycorrhizal fungi at the early stage of mycorrhiza formation

This work focuses on the functions of signaling molecules and secreted proteins released from mycorrhizal fungi at the early stage ofMycorrhiza formation, which helps host plants acquire more nutrients and water, thereby improving host plant resistance to abiotic and biotic stresses.

Characterization of the Trichoderma reesei hydrophobins HFBI and HFBII

Property and biological roles of the HFBI and HFBII hydrophobins produced by Trichoderma reesei were studied and effects of these hydrophOBins on the cultivation of T.Reesei in bioreactors were evaluated.

Heterogeneous expression and emulsifying activity of class I hydrophobin from Pholiota nameko

This work has attempted to express class I hydrophobin PNH1 from Pholiota nameko fused with glutathione S-transferase (GST) in Escherichia coli, and revealed that the purified GST–PNH1 had almost the same emulsifying activity as P NH1.

Phosphorous availability influences the dissolution of apatite by soil fungi

Apatite (Ca10(PO4)6(OH,F,Cl)2) is the primary inorganic source of phosphorus in the biosphere. Soil fungi are known to increase plant‐available phosphorus by promoting dissolution of various

Production and characterization of hydrophobins wirh potential for oral medicine applications

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Gene Expression during Pi Deficiency in Pholiota nameko: Accumulation of mRNAs for Two Transporters

The effects of Pi deficiency on gene expression in Pholiota nameko showed that these pdi genes were expressed in various patterns during Pi deficiency, and deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters.

Isolation and characterization of a cDNA from Catharanthus roseus which is highly homologous with phosphate transporter

The PIT1 gene was expressed in roots, stems, and young whole plant of C. roseus, but not in leaves, and Expression of the PIT1 cDNA enabled DpU to grow on LP medium.

LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato.

The isolation and characterization of a novel tomato phosphate starvation-induced gene (LePS2) representing an acid phosphatase represents a specific response to Pi starvation, and it is not affected by starvation of other nutrients or abiotic stresses.

Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea.

The results suggest that hydrophobins may have a role in the elaboration of infective structures by fungi and may fulfill other functions in fungal phytopathogenesis.

Cloning and characterization of two hydrophobin genes differentially expressed during fruit body development in Lentinula edodes.

Poor expression of these two hydrophobins in monokaryotic parents indicates that these two genes are under mating-type regulation, and it is suggested that differential expression ofThese two L. edodes hydrophobia during fruit development may contribute to their distinct roles in fruiting of this mushroom.

Structure of a cDNA for an acid phosphatase from phosphate-deficient lupin (Lupinus albus L.) Roots

Northern blot analysis revealed that the corresponding mRNA accumulated in both shoots and roots under phosphate deficient conditions, and shared a high homology with that of purple APases from germinated seeds of Phaseolus vulgaris and those secreted from Arabidopsis thaliana.

A phosphate-starvation inducible β-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family

We have previously isolated a phosphate starvation-response (psr) cDNA clone, psr3.1, from Brassica nigra which encodes a β-glucosidase. Southern blots of Arabidopsis thaliana genomic DNA probed with

Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus.

Divided root-system studies support the hypothesis that signal(s) for the Pi-starvation response may arise internally because of the changes in cellular concentration of phosphorus, and suggest that the epidermally localized Pi transporters may play a significant role in acquiring the nutrient under natural conditions.

Isolation and characterisation of five different hydrophobin-encoding cDNAs from the fungal tomato pathogen Cladosporium fulvum

Five different hydrophobin-encoding cDNA clones from Cladosporium fulvum were isolated from cDNA libraries, made from nutrient-depleted mycelium, and showed clear differences in expression patterns at various stages of development, and in liquid media lacking either carbon or nitrogen.