Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate-Mg2+ method.

@article{Harris1997ThreeGO,
  title={Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate-Mg2+ method.},
  author={N. Stuart Harris and V Galpchian and Jacob Thomas and Eugene L. Iannotti and Terence Law and Nader Rifai},
  journal={Clinical chemistry},
  year={1997},
  volume={43 5},
  pages={816-23}
}
We report on the analytical performance of three generations of HDL-cholesterol assays: phosphotungstic acid/Mg2+, Spinpro, and a homogeneous method, N-geneous. The run-to-run imprecision (CV) of all assays was < or = 4.9%, and all results correlated highly with those of a modified reference procedure (r > or = 0.96). At triglycerides concentrations < 4000 mg/L, these field methods showed an acceptable systematic error (y = 1.12x - 47, 1.05x - 23, and 0.96x + 8 for the phosphotungstate, Spinpro… CONTINUE READING

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