Three-dimensional visualization of the distribution, growth, and regeneration of monoaminergic neurons in whole mounts of immature mammalian CNS.

@article{Luque1998ThreedimensionalVO,
  title={Three-dimensional visualization of the distribution, growth, and regeneration of monoaminergic neurons in whole mounts of immature mammalian CNS.},
  author={Juan Mario Troyano Luque and Virginie Biou and John G. Nicholls},
  journal={The Journal of comparative neurology},
  year={1998},
  volume={390 3},
  pages={427-38}
}
At birth, the opossum, Monodelphis domestica, corresponds roughly to a 14-day-old mouse embryo. The aim of these experiments was to compare the distribution of monoaminergic neurons in the two preparations during development and to follow their regeneration after injury. Procedures that allowed antibody staining to be visible in transparent whole mounts of the entire central nervous system (CNS) were devised. Neurons throughout the brain and spinal cord were stained for tyrosine hydroxylase (TH… CONTINUE READING