Three-dimensional tumor models: Promoting breakthroughs in nanotheranostics translational research

  title={Three-dimensional tumor models: Promoting breakthroughs in nanotheranostics translational research},
  author={Ana Katrina Mapanao and Valerio Voliani},
  journal={Applied Materials Today},

From 2D to 3D Cancer Cell Models—The Enigmas of Drug Delivery Research

This review provides a comprehensive overview of the 3D cancer cell models currently available and discusses in which way each 3D model mimics the in vivo tumor physiology, how they can or have been used in nanomedicine research and to what extent the results obtained so far affect the progress of nanomediine development.

Tumor grafted – chick chorioallantoic membrane as an alternative model for biological cancer research and conventional/nanomaterial-based theranostics evaluation

The establishment and increased employment of such a model may resolve some of the limitations present in the standard preclinical tumor models, thereby redefining the preclinical research workflow.

Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology

The biggest challenge in this matter is the reproducibility of the MCTS and the associated full-scale characterization of this system’s components.

Monitoring and modulation of the tumor microenvironment for enhanced cancer modeling

In this review, conventional cancer models are summarized, and a primer on emerging techniques for monitoring and modulating the tumor microenvironment is presented and discussed.

A Standard Protocol for the Production and Bioevaluation of Ethical In Vivo Models of HPV-Negative Head and Neck Squamous Cell Carcinoma

A standardized and optimized protocol for the production and biocharacterization of human papillomavirus-negative head and neck chick chorioallantoic membrane models from a commercial cell line (SCC-25) is reported.

Doxorubicin-Loaded Gold Nanoarchitectures as a Therapeutic Strategy against Diffuse Intrinsic Pontine Glioma

This study evaluated the therapeutic efficacy of passionfruit like nanoarchitectures functionalized with human serum albumin and loaded with doxorubicin and found that NA-HSA-Dox were significantly effective at penetrating DIPG spheroids and subsequently at reducing the proliferation and colony formation in DipG cells.

Endogenously-Activated Ultrasmall-in-Nano Therapeutics: Assessment on 3D Head and Neck Squamous Cell Carcinomas

This work reports a straightforward protocol for the production of high throughput 3D models of negative or positive HPV-associated HNSCCs, together with their employment in the therapeutic evaluation of gold ultrasmall-in-nano architectures comprising an endogenously-activatable cisplatin prodrug.



Multicellular Tumor Spheroids (MCTS) as a 3D In Vitro Evaluation Tool of Nanoparticles.

MCTS are suitable models to gain additional information on nanoparticles while not being able to replace in vivo studies, they can bridge the gap between traditional 2D in vitro studies and in vivo models.

Impact of multicellular tumor spheroids as an in vivo-like tumor model on anticancer drug response

Findings indicate that the method of drug encapsulation in liposomes may improve the treatment efficacy of chemotherapeutic agents.

3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained

The analyses, carried out using a novel open source software capable of performing an automatic image analysis of 3D tumor colonies, showed that a number of morphology parameters affect the response of large spheroidids to treatment and indicate the need for a pre-selection of tumor spheroids of homogeneous volume and shape before use in a cytotoxicity test.

Mechanisms and Barriers in Cancer Nanomedicine: Addressing Challenges, Looking for Solutions.

This Nano Focus presents the main discussion topics and conclusions from a recent workshop: "Mechanisms and Barriers in Nanomedicine", on biological, toxicological, immunological, and translational aspects of nanomedicines and approaches to move the field forward productively.

Incorporation of multicellular spheroids into 3‐D polymeric scaffolds provides an improved tumor model for screening anticancer drugs

A novel in vitro model in which multicellular aggregates, or spheroids, were incorporated into 3‐D porous scaffolds showed that spheroid‐seeded scaffolds have much higher drug resistance than monolayer cultures, spheroid cultures on flat substrates,or scaffolds seeded with dispersed cells.

Production of Uniform 3D Microtumors in Hydrogel Microwell Arrays for Measurement of Viability, Morphology, and Signaling Pathway Activation.

The hydrogel microwell array platform can be applied successfully to generate hundreds of uniform microtumors within 3-6 days from many cervical and breast, as well as head and neck squamous cell carcinoma (HNSCC) cells and is demonstrated to provide more physiological in vitro 3D tumor models to investigate how tumor size influences signaling pathway activation and cancer drug efficacy.

Liquid-based three-dimensional tumor models for cancer research and drug discovery

Different tumor models are described and a model known as tumor spheroid is focused on, which addresses the influence of stromal fibroblasts and immune cells on cancer cells in tumor microenvironment, study cancer stem cells, and facilitate compound screening in the drug discovery process.

Cancer tissue engineering - new perspectives in understanding the biology of solid tumours - a critical review

This critical review of three-dimensional biomimetic tumour models focuses upon interesting studies recently reported in the published literature about cancer TE, grouping their findings by tumour type, to give a snapshot picture of the current achievements to those cancer scientists, who are wishing to approach the field of TE.