Three-dimensional tumor models: Promoting breakthroughs in nanotheranostics translational research

@article{Mapanao2020ThreedimensionalTM,
  title={Three-dimensional tumor models: Promoting breakthroughs in nanotheranostics translational research},
  author={Ana Katrina Mapanao and Valerio Voliani},
  journal={Applied Materials Today},
  year={2020},
  volume={19},
  pages={100552}
}
Abstract Three-dimensional tumor models are becoming extensively employed in cancer research due to their accessibility and close representation of physiological tumor conditions. Hence, novel oncological diagnostic, therapeutic and combined theranostic platforms, including nanomaterial-based systems, are increasingly evaluated at preclinical stage by means of such models. Here, we provide an overview of the functional features and methods of preparation of some of the most interesting 3D tumor… Expand

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References

SHOWING 1-10 OF 217 REFERENCES
3D tumor models: history, advances and future perspectives.
TLDR
3D tumor models have a long way to go before they are used commonly as in vitro tumor models in pharmaceutical industrial research, and application of techniques such as microfluidics, imaging and tissue engineering to improve the models are introduced. Expand
Multicellular Tumor Spheroids (MCTS) as a 3D In Vitro Evaluation Tool of Nanoparticles.
TLDR
MCTS are suitable models to gain additional information on nanoparticles while not being able to replace in vivo studies, they can bridge the gap between traditional 2D in vitro studies and in vivo models. Expand
Impact of multicellular tumor spheroids as an in vivo-like tumor model on anticancer drug response
TLDR
Findings indicate that the method of drug encapsulation in liposomes may improve the treatment efficacy of chemotherapeutic agents. Expand
3D tumor spheroids: an overview on the tools and techniques used for their analysis.
TLDR
The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids. Expand
Mechanisms and Barriers in Cancer Nanomedicine: Addressing Challenges, Looking for Solutions.
TLDR
This Nano Focus presents the main discussion topics and conclusions from a recent workshop: "Mechanisms and Barriers in Nanomedicine", on biological, toxicological, immunological, and translational aspects of nanomedicines and approaches to move the field forward productively. Expand
3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained
TLDR
The analyses, carried out using a novel open source software capable of performing an automatic image analysis of 3D tumor colonies, showed that a number of morphology parameters affect the response of large spheroidids to treatment and indicate the need for a pre-selection of tumor spheroids of homogeneous volume and shape before use in a cytotoxicity test. Expand
Incorporation of multicellular spheroids into 3‐D polymeric scaffolds provides an improved tumor model for screening anticancer drugs
TLDR
A novel in vitro model in which multicellular aggregates, or spheroids, were incorporated into 3‐D porous scaffolds showed that spheroid‐seeded scaffolds have much higher drug resistance than monolayer cultures, spheroid cultures on flat substrates,or scaffolds seeded with dispersed cells. Expand
Production of Uniform 3D Microtumors in Hydrogel Microwell Arrays for Measurement of Viability, Morphology, and Signaling Pathway Activation.
TLDR
The hydrogel microwell array platform can be applied successfully to generate hundreds of uniform microtumors within 3-6 days from many cervical and breast, as well as head and neck squamous cell carcinoma (HNSCC) cells and is demonstrated to provide more physiological in vitro 3D tumor models to investigate how tumor size influences signaling pathway activation and cancer drug efficacy. Expand
Liquid-based three-dimensional tumor models for cancer research and drug discovery
TLDR
Different tumor models are described and a model known as tumor spheroid is focused on, which addresses the influence of stromal fibroblasts and immune cells on cancer cells in tumor microenvironment, study cancer stem cells, and facilitate compound screening in the drug discovery process. Expand
Cancer tissue engineering - new perspectives in understanding the biology of solid tumours - a critical review
TLDR
This critical review of three-dimensional biomimetic tumour models focuses upon interesting studies recently reported in the published literature about cancer TE, grouping their findings by tumour type, to give a snapshot picture of the current achievements to those cancer scientists, who are wishing to approach the field of TE. Expand
...
1
2
3
4
5
...