Three-dimensional structure of the prolamellar body in squash etioplasts

  title={Three-dimensional structure of the prolamellar body in squash etioplasts},
  author={Shinji Murakami and Naoko Yamada and Misuzu Nagano and Masako Osumi},
SummaryHigh resolution scanning electron microscopy revealed that the basic unit of the paracrystalline network in squash prolamellar body is a tetrapodal structure, which has four short tubular arms meeting at one point with equal angle. Fractured faces of the prolamellar bodies displayed three lattice forms; hexagonal, square and zigzag (distorted hexagonal) lattices. Tilting observations of the ultrathin sections, together with scanning electron microscope observations, showed that the… 
Membrane geometry of “open” prolamellar bodies
The “open” type of prolamellar body in etiplasts was examined by electron microscopy to characterise its three-dimensional organisation, which is like that of the cages of water molecules in type IV clathrate-hydrates, point group P6/mmm, but about two orders of magnitude larger.
Electron tomography analysis of the prolamellar body and its transformation into grana thylakoids in the cryofixed Arabidopsis cotyledon
The para-crystalline structure of prolamellar bodies (PLBs) and light-induced etioplasts-to-chloroplasts transformation have been investigated with electron microscopy methods. However, these studies
Spatial Nano-Morphology of the Prolamellar Body in Etiolated Arabidopsis thaliana Plants With Disturbed Pigment and Polyprenol Composition
It is established that the addition of sucrose to the growing media significantly affected the size and compactness of the PLB, and an increased level of Pchlide (pif1) resulted in the substantial shift of the structural balance between outer and inner PLB water channels and overall PLB compactness compared to wild type plants.
Prolamellar-body structure, composition of molecular species and amount of galactolipids in etiolated, greening and reetiolated primary leaves of oat, wheat and rye
The results presented in this paper are not consistent with the hypothesis that the molar ratio of MGDG/DGDG is responsible for the tubular structure of prolamellar bodies in etioplasts.
Para-crystalline membrane structures resembling prolamellar bodies in the invasion zones of indeterminate root nodules of Vicia faba L.
It is proposed that the para-crystalline structures found in the invasion zones of indeterminate root nodules of Vicia faba serve as a reservoir of membranes for the formation of the numerous symbiosomes that propagate and fill the infected cells, and suggest naming them pro-symbiosome membrane bodies.
Single crystallization of an inverse bicontinuous cubic phase of a lipid.
  • T. Oka, Hiroki Hojo
  • Materials Science
    Langmuir : the ACS journal of surfaces and colloids
  • 2014
A single crystal region of an inverse bicontinuous cubic (QII) phase of a lipid, 1-monoolein is produced by a simple method by starting with the lipid of the sponge (L3) phase in the presence of 1,4-butanediol.
Prolamellar bodies of oat, wheat, and rye: Structure, lipid composition, and adsorption of saponins
The saponin adsorption is a peculiarity of oat PLBs but not a general tool for PLB-analysis, and experiments with the chelating agent EDTA indicate an external influence on thePLB construction and underline the idea that the PLB construction is not influenced by the membrane components themselves.
Chapter 6 Cubic Membranes
Disintegration of the prolamellar body structure at high concentrations of Hg2+.
The results suggest that Hg (2+) accelerates the disruption of the PLB membranes and that NADPH can only partially prevent this process.


Analytical Studies on the Structure of Prolamellar Body
The lattice structure of prolamellar body in the plastid of Phaseolus vulgaris cultured in the dark was stereometrically studied on a number of serial sections using electron microscope. The figures
Zur Kristallgitterstruktur der sogenannten Prolamellarkörper in Proplastiden etiolierter Bohnen
The spatial arrangement of tubules building up the prolamellar bodies of proplastids in etiolated bean leaves was investigated by electron microscopy. The basic unit of this arrangement is the
The greening process in plastids
Plastids in etiolatedAvena leaves were studied by electron microscopy of thin sectioned material fixed in glutaraldehyde and osmium tetroxide and embedded in Epon to conclude that this type of organisation is widespread in plants.
The membrane geometry of the prolamellar body
It is shown that in spite of the very different apparent morphology of these two membranous structures, their membranes have similar average curvature and inside-outside surface areas, which implies that the packing or molecular organization of the lipids and proteins can be similar in the two structures.
Zur Kristallgitterstruktur der sogenannten Prolamellarkörper in Proplastiden etiolierter Bohnen
The non-concentric prolamellar bodies of proplastids isolated from etiolated bean leaves were investigated by electron microscopy. The arrangement of tubules in a number of prolamellar bodies or in
On the significance of prolamellar bodies in membrane development of etioplasts
PLBs are seen as a secondary product of membrane formation in darkness, while the PT-membranes possess the apparatus for a fast greening process.
On the Lipid Like Character of Prolamellar Bodies
In dark grown plants the etioplasts show an interesting internal structure: the “prolamellar body” (PLB). Many attempts have been made to explain its paracrystalline structure, and there have been
A Comparison between Prolamellar Bodies and Prothylakoid Membranes of Etioplasts of Dark-Grown Wheat Concerning Lipid and Polypeptide Composition.
It is concluded that prolamellar bodies are formed when monogalactosyl diacylglycerol is present in larger amounts than can be stabilized into planar bilayer prothylakoid membranes by lamellar lipids or proteins.
Characterization of prolamellar bodies and prothylakoids fractionated from wheat etioplasts
The results strongly indicate the presence of phototransformable protochlorophyllide in the prolamellar bodies proper, while the main bulk of proteins are located in the prothylakoids.