Three-dimensional structure of the crystalloid in the microbody of Kloeckera sp.: composite crystal model

  title={Three-dimensional structure of the crystalloid in the microbody of Kloeckera sp.: composite crystal model},
  author={Masako Osumi and Misuzu Nagano and Naoko Yamada and Jun Hosoi and Mitsuhiro Yanagida},
  journal={Journal of Bacteriology},
  pages={376 - 383}
Electron microscopic investigations using the cryosectioning technique, together with electron diffraction, optical diffraction, and computer simulation, were carried out for the determination of the intrinsic structure of the crystalloid in the microbody of Kloeckera sp. The lattice images seen in the cryosections could be changed from one to another by tilting the specimen at an appropriate angle, the images obtained being well consistent with those obtained by computer simulation. The… 
Architecture of peroxisomal alcohol oxidase crystals from the methylotrophic yeast Hansenula polymorpha as deduced by electron microscopy
The architecture of alcohol oxidase crystalloids occurring in vivo in the peroxisomes of methylotrophic yeasts was deduced from electron micrographs of similar crystals of the Hansenula polymorpha
Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Kloeckera and Saccharomyces cerevisiae Yeast Cells
In the cytological analysis of the yeasts, intracellular structures were well preserved by using the freeze-substitution fixation method, and the previous model of a yeast cell was modified.
Visualization of yeast cells by electron microscopy.
  • M. Osumi
  • Biology
    Journal of electron microscopy
  • 2012
The dynamic process of cell wall formation was clarified through the study of the protoplasts, and it was found that β-1,3-glucan, β,1,6- glucan and α-1+, as well as α-galactomannan, are ingredients of the cell wall.
Properties of catalase purified from a methanol-grown yeast, Kloeckera sp. 2201.
Catalase, a marker enzyme of peroxisomes, was purified to homogeneity from whole cells of Kloeckera sp. 2201 (a strain of Candida boidinii) grown on methanol by means of ammonium sulfate
Cytochemical study of catalase activity in Candida boidinii during incubation on methanol
Not only peroxisomes but also mitochondria may be structurally and functionally responsible for the high catalase activity of some methanol-assimilating yeasts and the contribution of the mitochondria to the utilization of meethanol may be significant.
Subcellular fractionation of a hypercellulolytic mutant, Trichoderma reesei Rut-C30: localization of endoglucanase in microsomal fraction
The growing mycelia of Trichoderma reesei Rut-C30 are richly endowed with endoplasmic reticula and a variety of pleomorphic sub cellular bodies, and fractionation in the presence of MgCl2 improved the preservation of subcellular bodies derived from the endoplASM.
Substrate utilization, non-carbohydrate substrates
Although there are more than 50 recognized genera of yeasts, only a few genera are capable of utilizing a non-carbohydrate substrate as their sole source of carbon and energy; these genera include
Autotrophic Microbiology and One-Carbon Metabolism
The biochemistry and Genetics of C1 Metabolism in the Pink Pigmented Facultative Methylotrophs and the Applications of Alcohol Oxidase from M methylotrophic Yeasts are studied.


Ultrastructure of methanotrophic yeasts
The data presented suggest that microbodies, and the catalase contained within them, play a role in eucaryotic methane metabolism.
Ultrastructure of methanol-utilizing yeast cells: appearance of microbodies in relation to high catalase activity
Nine strains of methanol-utilizing yeasts belonging to the genera Candida, Hansenula, Kloeckera, Pichia, and Torulopsis were examined with respect to the interrelationship between their catalase content and ultrastructure and several morphological differences were observed.
Electron microscopic studies revealed that these fractions were largely comprised of morphologically well-preserved microbodies with a smaller number of more or less degraded organelles, but were virtually devoid of mitochondria.
Development of Microbodies in the yeast Kloeckera growing on methanol
Though the number of microbodies did not change during prolonged cultivation, their size became larger with the passage of cultivation time, and the activities of catalase and alcohol oxidase were confirmed in the particulate fractions throughout the cultivation period.
Microbody of methanol-grown yeasts. Localization of catalase and flavin-dependent alcohol oxidase in the isolated microbody.
Localization of a flavin-dependent alcohol oxidase as well as characteristic microbody enzymes (catalase and D-amino acid oxidase) were ascertained in the isolated microbodies, whereas formaldehyde and formate dehydrogenases were detected in the cytoplasmic region.
Catalase Activities of Hydrocarbon-utilizing Candida Yeasts
Cytochemical studies using 3,3′-diaminobenzidine (DAB) revealed that theCatalase activity was located in microbodies, suggesting that the catalase activities would be related to the hydrocarbon metabolism in the yeasts.
Thirty-one freshly isolated strains of methanol-assimilating yeasts were identified as Pichia cellobiosa, Candida boidinii,candida koshuensis, and Torulopsis molischiana, which showed characteristics similar to those of PichIA lindnerii, and the chemotaxonomic characteristics of the two were in good agreement.
Dissoziation der Rinderleber-Katalase in ihre Untereinheiten
Earlier results from other laboratories have shown that beef liver catalase is built up of subunits. However there was no integral relationship between the number of subunits and the number of active