Three-dimensional structure of renal Na,K-ATPase from cryo-electron microscopy of two-dimensional crystals.

@article{Hebert2001ThreedimensionalSO,
  title={Three-dimensional structure of renal Na,K-ATPase from cryo-electron microscopy of two-dimensional crystals.},
  author={Hans Hebert and Pasi Purhonen and Henrik Vorum and Karen E Thomsen and Arvid B. Maunsbach},
  journal={Journal of molecular biology},
  year={2001},
  volume={314 3},
  pages={479-94}
}
The structure of Na, K-ATPase was determined by electron crystallography at 9.5 A from multiple small 2-D crystals induced in purified membranes isolated from the outer medulla of pig kidney. The density map shows a protomer stabilized in the E(2) conformation which extends approximately 65 A x 75 A x 150 A in the asymmetric unit of the P2 type unit cell. The alpha, beta, and gamma subunits were demonstrated in the membrane crystals with Western blotting and related to distinct domains in the… CONTINUE READING
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