Three-dimensional structure of low density lipoproteins by electron cryomicroscopy.

  title={Three-dimensional structure of low density lipoproteins by electron cryomicroscopy.},
  author={Elena V Orlova and Michael P. Sherman and Wah Chiu and Hiro-omi Mowri and L Courtney Smith and Antonio M. Gotto},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={96 15},
Human low density lipoproteins (LDL) are the major cholesterol carriers in the blood. Elevated concentration of LDL is a major risk factor for atherosclerotic disease. Purified LDL particles appear heterogeneous in images obtained with a 400-kV electron cryomicroscope. Using multivariate statistical and cluster analyses, an ensemble of randomly oriented particle images has been subdivided into homogeneous subpopulations, and the largest subset was used for three-dimensional reconstruction. In… CONTINUE READING