Three-dimensional structure of Na,K-ATPase determined from membrane crystals induced by cobalt-tetrammine-ATP.

@article{Skriver1992ThreedimensionalSO,
  title={Three-dimensional structure of Na,K-ATPase determined from membrane crystals induced by cobalt-tetrammine-ATP.},
  author={Elisabeth Skriver and U Kav{\'e}us and H Hebert and Arvid B. Maunsbach},
  journal={Journal of structural biology},
  year={1992},
  volume={108 2},
  pages={176-85}
}
The three-dimensional structure of Na,K-ATPase has been analyzed with electron microscopy and image processing. The enzyme, purified from pig kidney outer medulla, was arranged in a new form of tetragonal two-dimensional membrane crystals after incubation with cobalt-tetrammine-ATP, a stable MgATP complex analogue. Each continuous protein domain, as delineated by negative stain, consists of two alpha beta-protomers related by a dyad axis. The two rod-like regions are connected by a bridge… CONTINUE READING