Three-dimensional redox image of the normal gerbil brain.

  title={Three-dimensional redox image of the normal gerbil brain.},
  author={Akihiko Shiino and Munetaka Haida and Bertrand Beauvoit and Britton Chance},
  volume={91 4},
Regional differences in the redox ratio were studied in the gerbil brain. Brains were frozen using an in situ funnel-freezing method, and sliced coronally for scanning of mitochondrial redox imaging. The relative local redox ratio of nicotinamide-adenosine dinucleotide to its reduced form was calculated from fluorescence signals of intrinsic fluorochromes, i.e. reduced nicotinamide-adenosine dinucleotide and flavoproteins, using a high resolution fluorometer developed in our laboratory. Twelve… CONTINUE READING


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