Three-dimensional reconstruction using transmission electron microscopy reveals a swollen, bell-shaped structure of transient receptor potential melastatin type 2 cation channel.

@article{Maruyama2007ThreedimensionalRU,
  title={Three-dimensional reconstruction using transmission electron microscopy reveals a swollen, bell-shaped structure of transient receptor potential melastatin type 2 cation channel.},
  author={Yuusuke Maruyama and Toshihiko Ogura and Kazuhiro Mio and Shigeki Kiyonaka and Kenta Kato and Yasuo Mori and Chikara Sato},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 51},
  pages={36961-70}
}
Transient receptor potential melastatin type 2 (TRPM2) is a redox-sensitive, calcium-permeable cation channel activated by various signals, such as adenosine diphosphate ribose (ADPR) acting on the ADPR pyrophosphatase (ADPRase) domain, and cyclic ADPR. Here, we purified the FLAG-tagged tetrameric TRPM2 channel, analyzed it using negatively stained electron microscopy, and reconstructed the three-dimensional structure at 2.8-nm resolution. This multimodal sensor molecule has a bell-like shape… CONTINUE READING

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