In order to suit the needs of studies in spinal column surgery on three-dimensional spatial structure and morphology of lumbar-sacral canal and the nervous tissue within it, an experimental model was applied by the authors using lumbosacral specimens from two fresh young adult cadavers. Successive 2mm thick CTM screening was carried out from L3 to S2 vertebral bodies with an interval of 1mm. The serial two-dimensional CT photographical pictures so obtained were inputted into a computer and the three-dimensional images were reconstructed through a VIDAS image analysis system. The experimental results indicated that the three-dimensional images of reconstructed vertebral canal and the nervous tissue within it were lifelike. These images could not only reveal their three-dimensional structure and morphology, but also be cut and composed together at any direction and section by turning and transpositioning along X, Y and Z axes. The authors hold that the visual effect expressed by three-dimensional images reconstructed from two-dimensional pictures can play important role in stereomorphologic, biomechanical, and other studies in fields of anatomy, image analysis, and clinical medicine.