Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation.

@article{Chan2010ThreedimensionalPO,
  title={Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation.},
  author={Vincent Chan and Pinar Zorlutuna and Jae Hyun Jeong and Hyunjoon Kong and Rashid Bashir},
  journal={Lab on a chip},
  year={2010},
  volume={10 16},
  pages={
          2062-70
        }
}
Cell-encapsulated hydrogels with complex three-dimensional (3D) structures were fabricated from photopolymerizable poly(ethylene glycol) diacrylate (PEGDA) using modified 'top-down' and 'bottoms-up' versions of a commercially available stereolithography apparatus (SLA). Swelling and mechanical properties were measured for PEGDA hydrogels with molecular weights (M(w)) ranging from 700 to 10 000 Daltons (Da). Long-term viability of encapsulated NIH/3T3 cells was quantitatively evaluated using an… 
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TLDR
With rapid 2D fabrication and 3D assembly, NIH/3T3 cells encapsulated in hydrogels can be survived for over 14 days in 2D micromodules and 2 days in 3D microstructures with incubation, and it is shown that cells viability increased by decreasing PEGDA concentration.
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This chapter gives an overview of the current state-of-art of materials and stereolithographic techniques to produce constructs for tissue regeneration, outlining challenges for future research.
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