Three-dimensional model for the isolated recombinant influenza virus polymerase heterotrimer

  title={Three-dimensional model for the isolated recombinant influenza virus polymerase heterotrimer},
  author={Eva Torreira and Guy Schoehn and Yolanda Blanco Fern{\'a}ndez and N{\'u}ria Jorba and Rob W. H. Ruigrok and Stephen Cusack and Juan Ort{\'i}n and Oscar Llorca},
  booktitle={Nucleic acids research},
The genome of influenza A virus is organized into eight ribonucleoprotein complexes (RNPs), each containing one RNA polymerase complex. This RNA polymerase has also been found non-associated to RNPs and is possibly involved in distinct functions in the infection cycle. We have expressed the virus RNA polymerase complex by co-tranfection of the PB1, PB2 and PA genes in mammalian cells and the heterotrimer was purified by the TAP tag procedure. Its 3D structure was determined by electron… CONTINUE READING


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