Three-dimensional imaging of cerebellar mossy fiber rosettes by ion-abrasion scanning electron microscopy.

@article{Kim2013ThreedimensionalIO,
  title={Three-dimensional imaging of cerebellar mossy fiber rosettes by ion-abrasion scanning electron microscopy.},
  author={Hyun-Wook Kim and Namkug Kim and Ki Woo Kim and Im Joo Rhyu},
  journal={Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada},
  year={2013},
  volume={19 Suppl 5},
  pages={172-7}
}
The detailed knowledge of the three-dimensional (3D) organization of the nervous tissue provides essential information on its functional elucidation. We used serial block-face scanning electron microscopy with focused ion beam (FIB) milling to reveal 3D morphologies of the mossy fiber rosettes in the mice cerebellum. Three-week-old C57 black mice were perfused with a fixative of 1% paraformaldehyde/1% glutaraldehyde in phosphate buffer; the cerebellum was osmicated and embedded in the Araldite… CONTINUE READING