Three-dimensional heteronuclear NMR studies of RNA

@article{Nikonowicz1992ThreedimensionalHN,
  title={Three-dimensional heteronuclear NMR studies of RNA},
  author={Edward P Nikonowicz and Arthur Pardi},
  journal={Nature},
  year={1992},
  volume={355},
  pages={184-186}
}
MULTIDIMENSIONAL heteronuclear NMR has revolutionized solution structure determinations of proteins1-3. But this technique has not been applied to nucleic acids because of difficulties in the synthesis of isotopically (13C and/or 15N) labelled molecules. Here we report the application of three–dimensional heteronuclear NMR to the study of a uniformly 13C/15N or 15N–labelled RNA duplex of defined sequence. These experiments simplify resonance assignment and the analysis of proton–proton nuclear… CONTINUE READING

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