Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes

Abstract

The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D 'object plane'. Existing technologies, such as confocal or light sheet fluorescence microscopy have to utilize mechanical scanning, a time-multiplexing process, to capture a… (More)
DOI: 10.1038/srep31445

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