Three-dimensional elemental mapping of phosphorus by quantitative electron spectroscopic tomography (QuEST).

@article{Aronova2007ThreedimensionalEM,
  title={Three-dimensional elemental mapping of phosphorus by quantitative electron spectroscopic tomography (QuEST).},
  author={Maria A. Aronova and Young Chul Kim and Robert Harmon and Alioscka A. Sousa and Guofeng Zhang and Richard Leapman},
  journal={Journal of structural biology},
  year={2007},
  volume={160 1},
  pages={35-48}
}
We describe the development of quantitative electron spectroscopic tomography (QuEST), which provides 3-D distributions of elements on a nanometer scale. Specifically, it is shown that QuEST can be applied to map the distribution of phosphorus in unstained sections of embedded cells. A series of 2-D elemental maps is derived from images recorded in the energy filtering transmission electron microscope for a range of specimen tilt angles. A quantitative 3-D elemental distribution is then… CONTINUE READING
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