Three-dimensional electron microscopy of the reverse gyrase from Sulfolobus tokodaii.

  title={Three-dimensional electron microscopy of the reverse gyrase from Sulfolobus tokodaii.},
  author={Kyoko Matoba and Kouta Mayanagi and Syo Nakasu and Akihiko Kikuchi and K. Yamada H. Morikawa},
  journal={Biochemical and biophysical research communications},
  volume={297 4},
Reverse gyrase is a type IA topoisomerase, found in various hyperthermophiles and promotes ATP-dependent positive supercoiling of DNA. Electron microscopy combined with single particle analyses revealed the three-dimensional structure of the DNA-free Sulfolobus tokodaii reverse gyrase and two-dimensional average images of both the protein alone and that complexed with double-stranded DNA. The 23A resolution map exhibited a parallelogrammatic morphology of 110 x 87 x 43A, which is in good… CONTINUE READING

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